Chk1 antibody
WebChk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). WebSep 7, 2024 · Chk1 Antibody (G-4) is a mouse monoclonal IgG 2a κ Chk1 antibody, cited in 800 publications, provided at 200 µg/ml. raised against amino acids 1-476 representing full length Chk1 of human origin. …
Chk1 antibody
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WebMonoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. WebApr 14, 2024 · Abstract. The concept of “BRCAness” was first described in 2004 to define the situation in which a homologous recombination repair (HRR) defect in a tumor relates to and phenocopies BRCA1 or BRCA2 loss-of-function mutations. Soon after the discovery of synthetic lethality of PARP1/2 inhibitors in BRCA1- or BRCA2-deficient cells, McCabe …
WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free … WebChk1 kinase acts downstream of ATM/ATR kinase and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression (1). Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296.
WebCHK1 (CHEK1) is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. CHK1 can also phosphorylate p53 at Ser20 in vitro. Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. WebCheckpoint kinase 1, or Chk1, is a protein serine/threonine kinase that is an essential component of the ATR pathway, which links DNA abnormalities and double strand breaks to downstream signaling events. It is activated through phosphorylation by ATR at serine residues 345 and 317. Once activated, Chk1 phosphorylates downstream effectors of S ...
WebThe Chk1 Antibody confirms silencing of Chk1 expression, and the p42 MAPK Antibody was used to control for loading and specificity of Chk1 siRNA. Western blot analysis of extracts from 293, Mv1Lu, C6, HeLa …
WebChk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in … porsche reset toolWebProduct name Anti-Chk1 (phospho S296) antibody [EPR915] See all Chk1 primary antibodies Description Rabbit monoclonal [EPR915] to Chk1 (phospho S296) Host species Rabbit Tested applications Suitable for: WB more details Unsuitable for: ICC/IF or IHC-P Species reactivity Reacts with: Human Immunogen Synthetic peptide. porsche responsibilityWebHuman Phospho-Chk1 (S345) Antibody Summary. Detects human, mouse, and rat Chk1 when phosphorylated at S345. This antibody does not recognize Chk1 when … porsche reproduction wheelsWebMonoclonal Antibody for studying Chk1. Cited in 431 publications. Validated for Western Blotting, Simple Western™. Highly specific and rigorously validated in-house, Chk1 … irish cream cake recipe using cake mixWeb20 hours ago · In addition, an anti–IL-8 antibody improved inflammation and fibrosis in a monkey model of endometriosis , ... H. Zhao, Dominant mutations in CHK1 cause pronuclear fusion failure and zygote arrest that can be rescued by CHK1 inhibitor. Cell Res.31, 814–817 (2024). Crossref. porsche residencesWebThus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage. This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature. porsche restaurant californiaWebDetects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317. Source Polyclonal Rabbit IgG Purification Antigen Affinity-purified Immunogen Phosphopeptide containing human Chk1 S317 site Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. porsche residences miami