WebOct 1, 2013 · Second, the FMP probe formulation from Molecular Devices proposed for this assay is one of the most sensitive reagents among those commercially available, as determined through independent testing. 15 Most of the assays to monitor CFTR function that have been reported to date require considerable technical expertise and/or sample … WebWe explored the utility of the real-time FLIPR Membrane Potential (FMP) assay as a method to assess kappa opioid receptor (KOR)-induced hyperpolarization. The FMP Blue dye was used to measure fluorescent signals reflecting changes in membrane potential in KOR expressing CHO (CHO-KOR) cells.
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WebOct 1, 2001 · A fluorescence-based assay using the FLIPR Membrane Potential Assay Kit (FMP) was evaluated for functional characterization and high throughput screening (HTS) of potassium channel (ATP-sensitive K + channel; K ATP) modulators.The FMP dye permits a more sensitive evaluation of changes in membrane potential with a more rapid response … WebWe explored the utility of the real-time FLIPR Membrane Potential (FMP) assay as a method to assess kappa opioid receptor (KOR)-induced hyperpolarization. The FMP … broken arrow t shirt printing urbandale ia
Functional characterization of 5-HT1 - ScienceDirect
WebJul 28, 2007 · The synthesized compounds 8a-l have been evaluated as ligands for the alpha1beta2gamma2S GABAA receptors and the rho1 GABAC receptors using the FLIPR membrane potential (FMP) assay and by electrophysiology techniques. None of the tested compounds displayed activity at the GABAA receptors at concentrations up to 1000 microM. WebThe FMP assay kit (Molecular Devices) uses an oxonol-derived voltage-sensitive indicator mixed with fluorescent quenchers, which absorb the emitted light of the extracellular dye, eliminating the washing step after … WebAug 3, 2024 · These compounds showed IC 50 values below 100 µM in the [3 H]GABA uptake assay at hBGT1 (Table 1). In the FMP assay, the compounds showed a concentration-dependent increase in the fluorescence ... broken arrow t shirt company