Phis2 酵母单杂

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Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 …

BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum

Webb1 apr. 2016 · 豆丁网是面向全球的中文社会化阅读分享平台，拥有商业,教育,研究报告,行业资料,学术论文,认证考试,星座 ... Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). how many kids does blippi have

中国科学院植物研究所酵母单杂交文库筛选客户案列展示 - 知乎

http://muchong.com/t-9775583-1-pid-2 http://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 Webb12 apr. 2024 · 方法：以启动子a构建到酵母单杂交启动子载体phis2，文库质粒为pgadt7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载 … howard pearl nj

pHIS2酵母单杂交质粒_价格-品牌-详情介绍_丁香通

Webb产品名称：pHIS2酵母单杂交质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was … Webb人机验证 how many kids does bode miller haveWebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ... how many kids does blake lively want

"Webb2 aug. 2024 · 酵母单杂交技术是在酵母双杂基础上发展而来的一种研究核酸-蛋白相互作用的工具，被广泛用于研究真核细胞内基因的表达调控，如鉴别DNA结合位点发现潜在的 … " - Phis2 酵母单杂

Phis2 酵母单杂

BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … Webb质粒名称：pHis2 出品公司：Clontech 质粒类型：酵母单杂交系统克隆方法：多克隆位点，限制性内切酶载体大小： 7207bp 原核抗性： Kanamycin (卡那霉素) 真核抗 …

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Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ... http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627

http://www.honorgene.com/product_list/carrier_library/123112.html Webb酵母单杂交体系是在酵母双杂交体系基本原理的基础上，通过观察酵母细胞内报告基因的表达状况，在酵母细胞内研究DNA与蛋白质之间的相互作用的一种技术。许多真核生物的 …

Webb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 …

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Webb一、酵母单杂交. 酵母单杂交技术是在酵母双杂交基础上发展而来的一种研究核酸-蛋白相互作用的工具，被广泛用于研究真核细胞内基因的表达调控，如鉴别DNA结合位点发现潜 … how many kids does blake griffin haveWebb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 . Description. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA … howard pease fan clubWebbY187-pHis2YeastOne-HybridMediaKit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基，性价比更高。培养基以非常方便使用的 … howard peasehttp://www.zoonbio.com/molecular/yeast-one-hybrid-technology.html how many kids does bob saget haveWebb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载体，两端的EcoRI和 SacI酶切位点，序列如下： GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA … how many kids does bob ewell havehttp://www.zoonbio.com/molecular/yeast-one-hybrid.html how many kids does bojo haveWebb6 sep. 2024 · 摘要：本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以 … how many kids does bo jackson have